Figure 2
From: Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
Downregulation of Olig2 decreased viability of melanoma cells. (a) Nuclear and cytoplasmic extracts were analyzed by western blot analysis using Olig2 (AF2418) antibody. (b) Cells were transfected with negative control (Con) or CRISPR/Cas9-Olig2 plasmid (CRISPR-Olig2) (1 μg and 2 μg, respectively). Western blot analysis of Olig2 protein expression was performed with Olig2 (H-10) antibody. Original images of blots were provided in supplementary file. (c) Cells were transfected with negative control (Con) or CRISPR/Cas9-Olig2 plasmid (CRISPR-Olig2) (1 μg and 2 μg, respectively). Cell viability was determined by EZ-Cytox assay 24 h, 48 h and 72 h after transfection respectively. Results are presented as percentages of the Con and the data were analyzed using Student's unpaired t-tests (*p < 0.05; **p < 0.01; ***p < 0.001 versus Con cells, n = 3). (d) 48 and 72 h after transfection, cell number was measured by trypan blue exclusion. Results are presented as percentages of the Con and the data were assessed using Student's unpaired t-tests (*p < 0.05; **p < 0.01; ***p < 0.001 versus Con cells, n = 3).
