Figure 4

Olig2 knockdown decreased migration and invasion of melanoma cells. Wound healing assay was performed to evaluate the migration abilities of (a) A375 cells and (b) 501mel cells after silencing of Olig2 expression. The cell migration into the wound was measured at 24 h and 48 h after scratch. Representative images (magnification, × 40) of cells were taken celenas digital imaging system. Scale bar: 100 µm. The migration was determined by the rate of cells filling the scratched area (*p < 0.05; **p < 0.01; ***p < 0.001 versus Con cells, n = 3). Transwell assay was performed to evaluate the migration ability of (c) A375, and invasion ability of (d) A375 and (e) 501mel cells after silencing of Olig2 expression. The cells which migrated or invaded through the membrane were stained with DAPI for visualization of the nuclei. Representative images and quantitative analysis of migrating or invading cells (*p < 0.05; **p < 0.01 versus Con cells, n = 3).