Figure 6
From: Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
Downregulation of Olig2 decreased MMP-2/-9 activities and MMP-1 expression. Gelatin zymography assay was performed with the conditioned media from (a) A375 and (b) 501mel cells transfected with negative control (Con) or CRSPR/Cas9-Olig2 plasmid (CRISPR-Olig2) followed by TGF-β (10 ng/ml) treatment. The fold-change in MMP-2 and MMP-9 activities was assessed by densitometry compared to Con group. The band intensity of MMP-2 and MMP-9 was quantified using NIH ImageJ software. (*p < 0.05; **p < 0.01versus Con group and #p < 0.05 versus TGF-β group, n = 3). (c) MMP-2 and MMP-9 mRNA were evaluated using each primer by RT-PCR. The band intensity was quantified using NIH ImageJ software and normalized relative to β-actin. Original images of gels were provided in supplementary file. (d) The concentration of MMP-1 was measured by ELISA using the supernatant of two melanoma cells. (**p < 0.01; ***p < 0.001 versus Con group, n = 3).
