Figure 7
From: Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
The MEK inhibitor (PD98059) and PI3K inhibitor (LY294002) decreased expression of Olig2. (a) A375 cells were treated with PD98059 for 3 h and 6 h. Protein expression of p-ERK, ERK, Olig2 (H-10) and Snail was detected by western blot analysis. (b) 501mel cells were treated with LY294002 for the indicated times. Protein expression of p-AKT, AKT, Olig2 (H-10) and Snail was detected by western blot analysis. (c) A374 and (d) 501mel cells were transfected with negative control (Con) and CRISPR/Cas9-Olig2 plasmid (CRISPR-Olig2). After 24 h transfection, western blot analysis was performed to identify the expression of p-ERK1/2, ERK, p-AKT and AKT. Original images of blots were provided in supplementary file.
