Figure 1 | Scientific Reports

Figure 1

From: A comparative study of Helicobacter pylori infection in hamsters experimentally infected with liver flukes Opisthorchis felineus, Opisthorchis viverrini, or Clonorchis sinensis

Figure 1

Quantification of H. pylori by quantitative RT-PCR in colorectal feces, stomach, and bile samples from uninfected hamsters and those infected with one of the liver fluke species. (A) Feces samples. (B) Bile samples. (C) Stomach samples. Tenfold dilutions of the pET plasmid carrying the H. pylori ureA gene were amplified in triplicate. A linear calibration curve was built to determine the mean starting amount of DNA in each sample and was found to have an R2 value of 0.999. Gene copy numbers (meaning H. pylori cell counts) per microgram of DNA were determined by means of the standard curve at a crossing point in a graph of log concentration. The cell concentration and amounts of DNA were calculated to ensure the same initial DNA amount in each sample to be analyzed.

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