Figure 2

S. stercoralis developmental stages have distinct transcript expression profiles. (A) S. stercoralis developmental stages examined by RNA-Seq are well-separated, and the three biological replicates group closely, by multidimensional scaling (MDS) analysis. (B) Numerous transcripts are significantly (log2 fold change > 1.0; FDR < 0.05) up-regulated (red) and down-regulated (blue) in free-living adult males, in comparison to free-living adult females, by mean-difference (MD) analysis. A subset of transcripts are significantly up-regulated in free-living adult males to a greater extent than transcripts are down-regulated. Plots were constructed using EdgeR v.3.32.0.