Figure 3 | Scientific Reports

Figure 3

From: Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice

Figure 3

Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on VLDLR in mice. Typical western blotting images of VLDLR and loading control (Actb and Tubulin) in the liver, heart, gastrocnemius muscle, and epididymal white adipose tissue (eWAT) (A,B,C,D, respectively), and the total amount of type 1 and type 2 VLDLR (E,F,G,H) obtained by digitizing the band density of western blotting in wild-type (WT) or Vldlr-deficient (KO) mice fed the control (20C) or protein-restricted (3C) diet. Photographs of two samples are shown, and a black line in the lower image of muscle VLDLR and tubulin delineate the boundary between the gels processed in parallel. The brightness and contrast of the images depicting liver VLDLR, eWAT VLDLR and muscle tubulin were adjusted equally across the entire image. Unmodified full-length photographs of the blots are shown in Figs. S1 and S6. Values are expressed as a relative value with the WT 20C group as 1 and the mean ± SEM, n = 10 per group. **P < 0.01, *P < 0.05 versus 20C.

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