Figure 3
(A) Stereospecific conversion of 2MeGlu enantiomers by human glutamine synthetase. Reaction was performed with 10 mM substrate and 5 ng/mL enzyme in the presence of 10 mM ATP, 20 mM MgCl2, 40 mM NH4Cl, 25 mM 2-mercaptoethanol in 100 mM imidazole pH 7.0 buffer18. (B) Conversion of rac-2MeGln by human glutaminase GLS1. Reaction was performed with 10 mM substrate and 5 ng/mL enzyme in phosphate pH 7.4 buffer19. (C) Relative concentrations of (R)- and (S)-[18O]2MeGlu generated in the GLS1 hydrolysis assay. Data are shown as mean ± SEM, n = 3.
