Figure 4
Cell metabolism of 2MeGlu and 2MeGln. Data are shown as mean ± SEM, n = 3. Horizontal arrows represent an uptake–conversion–release cycle. (A) Astrocyte uptake, amidation, and release of 2MeGlu and its amide derivative, 2MeGln. Total intra- and extracellular amounts of 2MeGlu or [1,2-13C]Glu (–COOH) and 2MeGln or [1,2-13C]Gln (–CONH2) were determined in cells and media. Primary astrocyte cultures were incubated in the presence of 1 mM of racemic or enantiopure 2MeGlu or l-Glu for 2 h at 37 °C along with cell-free controls. (B) Neuronal uptake, hydrolysis, and release of 2MeGln and its hydrolyzed counterpart 2MeGlu. Total intra- and extracellular amounts of 2MeGln (–CONH2) and 2MeGlu (–COOH) were determined in cells and media. Primary neuron cell cultures were incubated in the presence of 1 mM 2MeGln for 2 h at 37 °C along with cell-free controls.
