Figure 1
From: Facile isolation and analysis of sporopollenin exine from bee pollen
Fluorescence imaging of sunflower and rape pollen grains, with and without the intine wall. The samples were stained with calcofluor white. Pictures in the upper row were taken using mercury lamp excitation and a DAPI filter cube (see the Methods section), while the lower ones with brightfield imaging. Some exine apertures where the presence and absence of the intine can be best observed, are marked by yellow (on fluorescent image) and red (on brightfield image) arrowheads. Scale bars represent 40 μm. (a) Sunflower pollen grains P1-purified by Soxhlet extractions and an aqueous wash, without a P2-purification. Note the presence of the protruding intine portions. (b) Sunflower pollen grains P1-purified by Soxhlet extractions and an aqueous wash, and P2-purified by enzymatic treatment. Note the absence of the intine. (c) Sunflower pollen grains P1-purified by Soxhlet extractions and an aqueous wash, and P2-purified by TBPH. Note the absence of the intine. (d) Rape pollen grains P1-purified by Soxhlet extractions and an aqueous wash, without a P2-purification. (e) Rape pollen grains P1-purified by Soxhlet extractions and an aqueous wash, and P2-purified by TBPH. Note the absence of the intine.
