Table 3 Ratio of the dry matter content of bee pollen samples P1-purified by Soxhlet extractions and an aqueous was, before and after different P2 purifications (removal of the intine wall and the cellular debris).

From: Facile isolation and analysis of sporopollenin exine from bee pollen

 

Mixed P2 residue %

Rape P2 residue %

Sunflower P2 residue %

Hot 10% aq. KOH

29

18

31

RT 10% aq. KOH

26

18

30

Hot H3PO4

35

15

28

RT H3PO4

84

68

38

TBPH

28

12

32

  1. Classical protocols and a method using TBPH are compared.
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