Figure 4 | Scientific Reports

Figure 4

From: Activation of nuclear factor-kappa B by TNF promotes nucleus pulposus mineralization through inhibition of ANKH and ENPP1

Figure 4

Inhibition of NF-κB pathway diminishes TNF-induced mineralization. (a) Representative image of Alizarin red staining and spectrophotometric quantifications of the staining (1 week): pretreatment of TNAP cells for 1 h with CAPE diminished the TNF effect on mineral deposition in a dose-dependent matter (n = 4); (b) representative image of Alizarin red staining and spectrophotometric quantifications of the staining (2 weeks) (n = 6); (c) qPCR results: pretreatment of TNAP cells for 1 h with 5 µg/mL CAPE diminished the effect of TNF on ANKH and (d) ENPP1 gene expression (n = 4). Significance represents p < 0.05. Data analyzed using One-way ANOVA.

Back to article page