Table 2 List of oligonucleotides.
Gene | Gene ID | Amplicon size (bp) | Seq. forward (5′ > 3′) | Fwd Tm (°C) | Sequence reverse (5′ > 3′) | Rev Tm (°C) |
|---|---|---|---|---|---|---|
Bovine ANKH | 511800 | 128 | CCA TGT GGA TGA GTC AGT GG | 55 | GCA CAT CCA ACC AGG AAA CT | 55.4 |
Bovine ENPP1 | 615535 | 159 | AAT TGA GCG CTT GAC GTT CT | 55.6 | TCA GTG CTG TGC TTG AAT CC | 55.6 |
Bovine COL1A2 | 282188 | 69 | ACA TGC CGA GAC TTG AGA CTC A | 58.1 | GCA TCC ATA GTA CAT CCT TGG TTA GG | 57.3 |
Bovine COL2A1 | 497142 | 125 | GCT TCC ACT TCA GCT ATG GA | 54.4 | CAG GTA GGC AAT GCT GTT CT | 54.9 |
Bovine TNAP | 280994 | 291 | GCC GGG GGA CAT GCA GTA CG | 54.9 | GCC GGG GGA CAT GCA GTA CG | 54.8 |
Bovine TNF | 280943 | 165 | AGA GGG AAG AGT TCC CCA GG | 57.2 | CCT CAG CTT GAG GGT TTG CT | 57.3 |
Bovine ACAN | 280985 | 150 | GGG AGG AGA CGA CTG CAA TC | 57.3 | CCC ATT CCG TCT TGT TTT CTG | 54.4 |
Bovine RUNX2 | 536911 | 62 | AGT GAT TTA GGG CGC ATT CCT | 56.6 | GAG GGC CGT GGG TTC TG | 57.3 |
Bovine COL10A1 | 282416 | 225 | GGA AAA CAA GGG GAG AGA GG | 54.5 | TCC CCT TTC TGT CCA TTC AG | 53.8 |
Bovine MMP13 | 281 | 104 | TCC AGT TTG CAG AGA GCT ACC | 56.6 | CTG CCA GTC ACC TCT AAG CC | 57.2 |
Human ANKH | 914 | 128 | CCA TGT GGA TGA GTC AGT GG | 55 | GCA CAT CCA ACC AGG AAA CT | 55.4 |
Human ENPP1 | 5167 | 150 | AAT TGA GCG CTT GAC GTT CT | 55.6 | TCA GTG CTG TGC TTG AAT CC | 55.6 |
Human TNAP | 249 | 275 | GGA CAT GCA GTA CGA GCT GA | 56.9 | CCA CCA AAT GTG AAG ACG TG | 45.5 |
