Figure 2
From: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells
NMDA receptor expression by HPASMCs in vivo. Human lung tissue containing pulmonary artery was stained with NMDA receptor subunit specific antibodies. PECAM-1 was used as a marker for vascular endothelial cells (EC). Antibody against PECAM-1 revealed immunoreactivity within the interior surface of pulmonary artery. Immunoreactive staining for both GluN1 (B) and four GluN2 subunits (2A–D) (C–F) was observed in pulmonary artery smooth muscle cells (SMC). Control experiments with primary antibody omitted showed no immunoreactivity in either pulmonary artery endothelial cells or PASMCs (G). Scale bar = 50 μM.
