Figure 4
From: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells
Full-length NMDA receptors are expressed by cultured HPASMCs. Cultured HPASMCs were lysed and immunoprecipitated with GluN1- or GluN2- subunit specific antibodies or control non-immune IgG, and the pellets were analyzed by Western blot. Immunopositive bands containing full-length GluN1 (A) and GluN2 (2A–D) (B–E) protein were detected in immune pellets from lysates of PASMCs but not in the IgG control conditions studied in parallel. Cropped images are shown for conciseness. Full-length blots are represented in Supplementary Fig. S9. Results are representative of three separate experiments.
