Figure 5

Tissue staining of retinal capillary layers at baseline (A) and 90 mmHg of IOP (B). The tissue area examined by OCTA was confirmed in gross specimens (upper left) and then lectin staining was performed. The entire tissue was divided into 25 tiles and orthogonal confocal images (× 5 magnification) of the lectin stain (upper middle) and RBC autofluorescence (upper right) were obtained. (second and third rows) En face view of the different capillary levels of the pig retina (upper: lectin-stained images, lower: RBC autofluorescence images).