Figure 1

Sigmoidal functions fit to experimental data and distributions of fitted parameters when the sigmoidal functions are fit to a population of infection events generated by Liu et al.¹⁶ without drug treatment. (A) Example of sigmoidal function fit to experimentally data of GFP intensity measured in arbitrary units (a.u.). A sigmodal curve (black line) is fitted to intensity observations from a single infection event (purple dots), allowing for the estimation of the midpoint (blue) and the maximum intensity value (orange). (B) Example of double-sigmoidal function fit to a single infection event where lysis is observed. A double sigmodal curve (black line) is fitted to intensity observations from a single infection event (purple dots), allowing for the estimation of the midpoint (blue), the maximum intensity value (orange), and the time point of lysis (green). (C) Distribution of slopes calculated at the midpoint. The slope is related to the viral replication rate. (D) Distribution of the maximum intensity. (E) Distribution of the amount of time until the half of the maximum intensity is reached. (F) Distribution of the length of infection time.