Figure 4

Estimates of mechanistic parameter posterior distributions in our model of PV infection from fitting the model to experimental data generated without drug treatment (Fig. 1C–F). Parameters are estimated by fitting the model described by the equations in Table 1 and illustrated in Fig. 2A. The parameters shown correspond to those labeled in each reaction. (A) Translation, which occurs in step 3 of the model. (B) Compartmentalization, a part of step 4. (C) Circularization, step 5. (D, E) Replication of positive and negative sense RNA, step 6. (F) Packaging, step 7. (G) The maximum number of compartments possible, considered in step 6. (H) The maximum number of replication cycles permitted by cellular resources, a limiting factor in step 4. (I) Consumption of the protein product 3A, step 4. (J) The probability for a newly synthesized genome to stay in the replication complex, step 8.