Figure 1
From: The antiviral effect of metformin on zika and dengue virus infection
Cytotoxicity (CC50), inhibition concentration (IC50), and selectivity index (SI) of MET in Huh-7 and U-87 cell lines infected with ZIKV. The CC50 on Huh-7 (A) and U-87 (B) cells were calculated with the vehicle (H2O) or increasing concentrations of MET (1, 2.5, 5, 7.5, 10, 15, 20, and 25 mM). The cell viability was evaluated by MTT assay at 24 h, and a linear regression analysis estimated the CC50. The IC50 was calculated for MET in ZIKV-infected Huh-7 (C) and U-87 (D) cells. Both cell lines were treated with MET (1, 2.5, 5, 7.5, 10, 15, 20, and 25 mM) or vehicle (H2O) for 24 h. The reduction of infection by MET was evaluated by flow cytometry, and the IC50 was estimated by linear regression analysis. The results were plotted with Graph Pad Prism software version 6.0.
