Figure 2

Met reduces viral yield and viral protein synthesis during the ZIKV infection in Huh-7 and U-87 cells. The Huh-7 and U-87 infected cells were treated with Met (1,7.5,15 mM) for 24 h. (A) Infected cells analyzed by flow cytometry. (B) Viral titers of the collected supernatants analyzed by foci forming units (FFU) assay. The NS3-ZIKV protein was analyzed by western blot in (C) Huh-7 cells, and (D) U-87 cells; the densitometric analysis is shown below. (E) Statistical analysis of densitometry between both cell lines. The results are presented in percentages considering as 100% the infected cells untreated (Control). The graphs represent the means ± SD of n = 3 independent experiments realized by duplicated. *p = 0.0332, **p = 0.0021, ***p = 0.0002 ****p < 0.0001.