Figure 4
AIM2 inhibits GC cell proliferation and migration in an AKT-dependent manner. (a,b) Immunoblotting of the indicated proteins in wild-type AGS and MKN45 cells (NC) and in cells with stable knockdown of AIM2 (KD) with or without treatment of Ly294002 (a, 20 μM) or MK2206 (b, 5 μM). DMSO treatment is considered as the control group. GAPDH as a loading control. (c) Immunoblotting of the indicated proteins in wild-type SGC7901 and MGC803 cells (VEC) and in cells with stable overexpression of AIM2 (OE). GAPDH as a loading control. (d) Immunoblotting of caspase-1 in AGS cells (NC vs. KD) and SGC7901 cells (VEC vs. AIM2). GAPDH as a loading control. (e) Colony formation assays were performed in AGS cells (NC vs. KD) in the presence or absence of Ly294002, and the relative number of colonies was quantified (right). Error bars, mean ± SEM from three biological replicates. (f) Migration assays were performed in AGS cells (NC vs. KD) in the presence or absence of Ly294002, and the migratory cells number was quantified (right). Error bars, mean ± SEM from three biological replicates. The proteins were quantified using the ImageJ software (version: 1.4.3). *P < 0.05; **P < 0.01; ***P < 0.001. The original Western blots are included in the Supplementary Fig. S3.
