Figure 5

Liver stains after 32 weeks of nutritional intervention study. (A) H&E staining (40 ×). (B) Sirius Red staining (40 ×). (C) GATA 4 staining (40×). (D) Quantification of hepatic fibrosis by Sirius Red. (E) Quantification of total liver collagen (µg /mg liver protein) in liver homogenates. (F) Quantification of positive HSC per field. Values represent the means ± SEM (n = 10). *p < 0.05 LFD vs HFDs groups; different letters indicate significant differences between HFD groups (p < 0.05). Scale bars = 100 µm.