Table 2 Dynamic range of the ddPCR-based assay. Detecting the Staphylococcus aureus ATCC25923 nucleic acid template by ddPCR system.
Counts of bacterial nucleic acid template | 31 pg | 3.1 pg | 310 fg | 0 |
---|---|---|---|---|
Theoretical copy number | 13,000 | 1300 | 130 | 0 |
Logarithmic transformation | 4.1139 | 3.1139 | 2.1139 | – |
Actual number of copies | 11,120 | 1058 | 96 | 0 |
Logarithmic transformation | 4.0461 | 3.0245 | 1.9823 | – |