Table 3 Antibody panel.
Antigens | CD3 | CD4 | CD8 | CD11c | CD14 | CD16 | CD20 | CD56 | CD159a | HLA-DR | Live/Dead |
|---|---|---|---|---|---|---|---|---|---|---|---|
Lymphocyte | CD3 | CD4 | CD8 | – | – | – | – | – | – | – | L/D |
NK-cell | – | – | CD8 | – | – | CD16 | – | CD56 | CD159a | Dim/– | L/D |
Monocyte | – | – | – | – | CD14 | CD16 | – | – | – | HLA-DR | L/D |
mDC | – | – | – | CD11c | – | CD16 | – | – | – | HLA-DR | L/D |
B-cell | – | – | – | CD11c | – | – | CD20 | – | – | – | L/D |
NKT Cell | CD3 | – | CD8 | – | – | CD16 | – | CD56 | – | – | L/D |
Fluorochromes | AF700 | PerCP-Cy5.5 | APC-Fire750 | PE | APC | BV786 | PE-Dazzle | BV605 | PE-Cy7 | BUV395 | Violet |
Clone | FN-18 | OKT4 | SK1 | 3.9 | M5E2 | 3G8 | 2H7 | MY31 | Z199 | G46-6 | N/A |
Manufacturer | BD | Biolegend | Biolegend | Biolegend | Biolegend | Biolegend | Biolegend | BD | Beckman Coulter | BD | Invitrogen |
