Figure 1

Pluripotency characteristics. This figure shows a phase-contrast photomicrograph of typical colonies for all 6 LND and all 6 control lines, along with immunostains for pluripotency markers (SSEA3, SSEA4, TRA-1-60, TRA-1-80, Nanog). All cultures were stained simultaneously, and all photomicrographs were taken using the same microscope settings. Scale bar is shown at bottom right and depicts 200 μM. Gene expression profiling shown as box plots at the bottom of the figure reveal that the control and LND lines expressed similar levels of genes associated with pluripotency.