Figure 4
Internalization of NEs in MDA-MB 231 cells. (a) Gallery of confocal microscopy images of MDA-MB 231 cells incubated with 150 µg/mL of C-NEs and ET-NEs labeled with TopFluor-PC (green channel) for 4 h at 37 ºC. (b) Representative confocal microscopy single-plan image of control vs C-NEs and ET-NEs internalization by MDA-MB 231 cells. (c) Fluorescence intensity (AU, arbitrary units) of two maximum projection images (resultant from the combination of all the sections shown in (a)) was determined using ImageJ software. Statistical analysis was performed using t test. P value ****p < 0.0001. Cell nuclei was stained with Hoechst (blue channel). Scale bars correspond to 25 µm.
