Figure 6

Temporal course of HIF-1α signaling during AKI to CKD transition. (A) mRNA levels of Hif1a, (B) mRNA levels of Vegfa, (C) Protein levels of HIF-1α, including a representative cropped blot, and (D) Protein levels of VEGF, including a representative cropped blot. Data are represented as the mean ± SE. n = 4, for S, UNx, and IR + UNx groups, in each studied period: 30, 60, 90, and 120 days post-ischemia. White circles represent the S, gray squares represent the UNx and black triangles represent the IR + UNx groups. The one-way analysis of variance (ANOVA) was used to determine statistical differences, using the Bonferroni correction for multiple comparisons. *p < 0.05 versus S group and + p < 0.05 versus UNx group in their respective period. Full-length blots are presented in Supplementary Figs. 8 and 9.