Table 2 qPCR primer sequences (rat).
Forward | Reverse | |
|---|---|---|
Cnb1 | 5′-TGCTTCAGGAGGGACTGACT-3’ | 5′-CCACCTCCCTCACACAAACT-3’ |
Glucagon | 5′-GAAGTTACCGCCCTGAGATT-3’ | 5′-CGCATTTATGACAAAGGGTTC-3’ |
Hprt | 5′-AGTCCCAGCGTCGTGATTAG-3’ | 5′-AATCCAGCAGGTCAGCAAAG-3’ |
Insulin2 | 5′-TGGGGAGCGTGGATTCTTCT-3’ | 5′-CAGAGGGGTGGACAGGGTAG-3’ |
MafB | 5′-TATTCCAAGGAGTCGCCAAG-3’ | 5′-CTGAGAGCCAGTGTTCACCA-3’ |
NeuroD1 | 5′GGATGATCAAAAGCCCAAGA-3’ | 5-GCAGGGTACCACCTTTCTCA-3’ |
Nfatc1 | 5′-TTGGATTCTGACGAGCTGTG-3’ | 5′-GTGCAGCTGGATCAAGAACA-3’ |
Nfatc2 | 5′-CATTCCCATCTGCAGCATCC-3’ | 5′-CCGTCCCGATGAAGATCTGA-3’ |
Notch1 | 5′-CTATGTTGTGGACCATGGCG-3’ | 5′-CGGCTTGCTGACATGACTTT-3’ |
Pax6 | 5′-AGGAACCAGAGAAGACAGGC-3’ | 5′-GTACGAGGAGGTCTGACTGG-3’ |
Scrt1 | 5′-ACATTCTCTTCGGCAGACCT-3’ | 5′-GGATGGCCCTTTGAGCAATG-3’ |
Syt4 | 5′-TACCAGCCATGGATGAACAATC-3’ | 5′-CAAAACTCAGGACGGTGAAGTG-3’ |
