Figure 2
Proliferative characteristics of LECs isolated from lung tissues. (a, b) Plots of proliferation when cultured in ECBM/5% FBS. (a) Cell growth was examined for 3 days and is expressed on the vertical axis as the relative ratio of absorbance (450 nm) compared with that on Day 0. LAM-LECs (T) (n = 4), LAM-LECs (V) (n = 3), and control LECs (n = 4). (b) Individual results of LAM-LECs (T) and control LECs at Day 3 were plotted. (c) Plot of proliferative responses for various growth factors. LECs were cultured in ECBM/5% FBS and stimulated for 3 days with VEGF-A (10 ng/mL), -C (50 ng/mL), -D (10 ng/mL), or a combination of 2–3 growth factors. Growth response is expressed on the vertical axis as the relative ratio of absorbance (450 nm) compared with that of each type of non-treated data (i.e., absorbance without growth factors). LAM-LECs (T) (n = 4), LAM-LECs (V) (n = 3), and control LECs (n = 4). Statistical significance was assessed using the Student’s t-test and one-way ANOVA followed by Tukey's multiple comparisons tests. *p < 0.05.
