Figure 3
Chamber migration assay of LECs isolated from lung tissues. (a) Representative microphotographs of LECs migrated to the lower side of membranes after incubation for 4 h. Membranes were stained with Diff-Quik (nuclei were stained as purple). (b) Plot of results from migration assay of LECs. LAM-LECs (T) (n = 4) and control LECs (n = 4). (c) Plot graph showing migration of LECs when stimulated by various growth factors. Chamber migration assays were performed with the addition of VEGF-A (10 ng/mL), -C (50 ng/mL), or -D (100 ng/mL). Responses are expressed on vertical axis as relative ratios of migrated cells compared with those of non-treated data (i.e., the number of migrated cells without added growth factors). LAM-LECs (T) (n = 4) and control LECs (n = 4). Statistical significance was assessed using the Student’s t-test and one-way ANOVA followed by Tukey's multiple comparisons test. *p < 0.05.
