Figure 6
The role of VEGFR-3 and integrin α9 in proliferation and migration of LECs. (a, b) Dot plot graphs of the effects of MAZ51 and the anti-integrin α9-neutralizing antibody (α9-Ab) on proliferation of LAM-LECs (T). LAM-LECs (T) were cultured in ECBM with 5% FBS for 3 days with the anti-integrin α9-neutralizing antibody (50 μg/mL) (a) or with MAZ51 (10 μM) (b). Growth response is expressed on the vertical axis as the relative ratio of absorbance (450 nm). (c-h) Dot plot graphs of the effects of MAZ51 and the anti- integrin α9-neutralizing antibody on migration of LAM-LECs (T). Chamber migration assays of LECs were performed using various grow factors: (c, d) VEGF-A (10 ng/mL); (e, f) VEGF-C (50 ng/mL); and (g, h) VEGF-D (100 ng/mL). Statistical significance was assessed using the Student’s t-test (a, b) or one-way ANOVA followed by Tukey’s multicomparison test (c-h). *p < 0.05, ** p < 0.01, *** p < 0.001 and n.s. = not significant. Abbreviations: α9-Ab = anti-integrin α9 neutralizing antibody.
