Figure 2

Scoring of subchondral bone changes in tibial plateau from BL/6^C57 and BL/6^NZB operated mice. Representative images of knee joints (right knee = SHAM; left knee = DMM) stained with Safranin O from BL/6^C57 (A) and BL/6^NZB (C) mice (magnification 10×). Scale bar, 500 μm. Subchondral bone thickening, number of trabeculae and osteophyte formation graded as normal (grade 0), mild (grade 1), moderate (grade 2) or severe (grade 3) were used as parameters to examine the subchondral bone of medial tibial plateau (MTP) in mouse knee joints. Quantification of bone changes in BL/6^C57 (B) and BL/6^NZB (D) mice at 8 weeks (BL/6^C57 = 9; BL/6^NZB = 10) following DMM is shown as subchondral bone score in medial tibial plateau (MTP). Comparison of bone changes score between BL/6^C57 and BL/6^NZB mice after DMM surgery (E). All data are shown as mean ± SEM; ***p < 0.001, **p < 0.01, *p < 0.05 by non-parametric Mann–Whitney test.