Figure 4

Changes in cartilage cellularity and cleaved caspase-3 expression in response to surgically OA induction. Images from Hematoxylin Eosin (H&E) stained sections of BL/6^C57 (A) and BL/6^NZB (C) mice knee joints (BL/6^C57 = 5; BL/6^NZB = 6). Original magnification 10×. Scale bar, 500 μm. Chondrocytes magnification (20×) is shown on the bottom-left corner of the images. Quantitative analysis of cartilage cell number in the medial compartment of BL/6^C57 (B) and BL/6^NZB (D) mice after DMM surgery. Comparison of cartilage cell number between DMM BL/6^C57 and DMM BL/6^NZB mice (E). Representative images of medial compartment of operated (DMM) knee joints from BL/6^C57 (left panel F) and BL/6^NZB (right panel F) stained with cleaved caspase-3 (CC3) antibody. Quantitative analysis of CC3 positive cells of knee joints from BL/6^C57(n = 4) and BL/6^NZB(n = 4) after DMM surgery (G). Original magnification 10×. Scale bar, 500 μm. Black arrow indicate positively stained chondrocyte. Graph represents means ± SEM. *p < 0.05 , **p < 0.01 by non-parametric Mann–Whitney test. Comparisons with significant differences are indicated; all other comparisons were non-significant.