Figure 5

Immunohistochemistry for LC3 and Beclin-1 were performed on mouse knee joint sections. Representative images of medial compartment of operated (DMM) knee joint from BL/6^C57 (left panel A) and BL/6^NZB (right panel A) stained with LC3. Quantitative analysis of LC3 positive cells of knee joints from BL/6^C57 and BL/6^NZB after DMM surgery (B). Comparison of the difference in LC3 positive cells between SHAM and DMM group (Δ) in joints from BL/6^C57 and BL/6^NZB mice (C). Representative images of medial compartment of operated (DMM) knee joints from BL/6^C57 (left panel D) and BL/6^NZB (right panel D) stained with Beclin-1 antibody. Quantitative analysis of Beclin-1 positive cells of knee joints from BL/6^C57 and BL/6^NZB after DMM surgery (E). Original magnification 10×. Scale bar, 500 μm. Black arrow indicate positively stained chondrocyte. Chondrocytes magnification (20×) is shown on the bottom-left corner of the images. Graphs represent means ± SEM; (BL/6^C57 = 4; and BL/6^NZB = 4). *p < 0.05, p = 0.0571 by non-parametric Mann–Whitney test.