Figure 3

The concentration–time profiles for 6 healthy volunteers (NO. 1–6) after they receive one subcutaneous injection of 50 mg etanercept. 5 mL blood sample was collected in heparinized centrifuge tubes at 2, 4, 8, 12, 24, 36, 48, 72, 120, 168, 336, 504, 672, 744, and 888 h. Serum samples were separately stored using the new (red symbols and line) or the conventional method (black symbols and line) until analysis. All samples were detected with the ELISA method.