Figure 2
From: Enhanced generation of influenza-specific tissue resident memory CD8 T cells in NK-depleted mice
NK depletion enhances the development of lung TRM following influenza infection. (A) C57BL/6 mice were infected with 103 PFU x31, administered anti-NK depleting antibody at −1, 1, and 3 dpi and rested for 45 days. TRM were identified as cells which did not positively stain with an anti-CD45 administered i.v. 3 min prior to euthanasia, previously gated on single cells, lymphocytes, CD8+CD44+NP-tet+ cells. The frequency (B), and number (C) of NP-tet+ TRM CD8+ T cells ± SEM recovered from the lung of NK depleted (grey) and sufficient (black) mice are depicted. (D) Representative expression of CD103 and CD69 by lung TRM cells. (E) Frequency of cells expressing CD103 or CD69 in NK-depleted (grey) or sufficient (black) mice. Graphs represent pooled data from 2 independent experiments using n ≥ 3 mice/group/time point (total of 6 mice per group/time point). *p < 0.05; unpaired Student’s t-test with Holm-Sidak multiple comparisons correction.
