Table 2 Optimized conditions for the coated PLA2R and the QD-labeled antibody.
Coating PLA2R | 0.5 mg/mL | 1 mg/mL | 2 mg/mL | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Standard serum (RU/mL) | 2 | 20 | 100 | 500 | 2 | 20 | 100 | 500 | 2 | 20 | 100 | 500 |
QDs-mAb dilution rate | ||||||||||||
1:200 | 247 | 1322 | 4376 | 10,175 | 306 | 1017 | 2539 | 6878 | 295 | 1185 | 2869 | 6769 |
1:100 | 249 | 2463 | 5668 | 10,477 | 292 | 2453 | 4400 | 10,533 | 258 | 2061 | 4489 | 8470 |
1:50 | 261 | 2342 | 5612 | 10,522 | 295 | 1222 | 2637 | 5553 | 337 | 1283 | 2721 | 5667 |
