Figure 2 | Scientific Reports

Figure 2

From: β2-adrenergic receptor agonist counteracts skeletal muscle atrophy and oxidative stress in uremic mice

Figure 2

IS administration caused a decrease in predominantly fast-twitch muscle fiber. (A, B) Immunohistochemical analysis of (A) fast (top, green) and slow (bottom, green) myosin heavy chain (MHC) isoforms counterstained with laminin (red) was performed for mouse gastrocnemius (GC) muscle cryosections obtained from mice with a unilateral nephrectomy after one-week treatment of vehicle (VEH) or indoxyl sulfate (IS) (left). The scale bar is 50 μm. The cross-sectional area and size distribution of fast and slow myofibers was quantitated (right). As a statistical analysis, a Cochran-Armitage trend test for fiber size distribution was performed. N = 4 mice per group. Error bars denote SEM. (B) Western blotting analysis of the fast and slow MHC isoforms was performed in GC muscle (left). Actin was utilized as a loading control. Quantitative densitometric data from western blots are shown (right). N = 4–5 mice per group. A full-length view of the membrane is shown in the supplementary Fig. 2. Error bars denote SEM. * p < 0.05, N.S.; no significance.

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