Figure 3
IS administration did not change the gene expression of proteolysis-related genes but suppressed protein synthesis (puromycin-labeled peptides). (A) qPCR analysis for mRNA expression of atrogin-1, MuRF-1, and myostatin in gastrocnemius (GC) muscle of unilaterally nephrectomized mice treated with vehicle (VEH) or indoxyl sulfate (IS). N = 5–6 mice per group. Error bars denote SEM. N.S.; no significance. (B) Western blotting using an anti-puromycin antibody was performed for analysis of GC muscle protein synthesis (puromycin-labeled peptides). The ponceau S staining was examined as a loading control. Quantitative densitometric data from western blots are shown (right). N = 2–3 mice per group. A full-length view of the membrane is shown in the supplementary Fig. 3. Error bars denote SEM. * p < 0.05, Puro; puromycin. #; p < 0.001 compared to VEH without IS treatment.
