Figure 2
From: Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
Cytoplasm to nucleus translocation of Rbm24 during myogenic differentiation in C2C12 cells. (a) Schematic representation of GFP-tagged full-length and truncated forms of Rbm24 proteins. Numbers indicate amino acid residues in mouse Rbm24 protein. (b) Western blot analysis of indicated fusion proteins in C2C12 myoblasts 24 h after transfection using a GFP antibody. Full-length blot is presented in Supplementary Fig. S1. (c–j) Subcellular localization of different fusion proteins after transient transfection in C2C12 cells, which were counterstained with DAPI. (c,d) GFP alone was uniformly distributed in proliferating myoblasts 24 h after transfection and in multinucleated myotubes after 3 days of differentiation. (e,f) GFP-Rbm24 was heterogeneously distributed in the cytoplasm of myoblasts, but was predominantly localized in the nucleus of differentiated myotubes. (g,h) GFP-Rbm24ΔRRM was mainly localized in the nucleus of myoblasts, and was distributed both in the cytoplasm and nucleus of differentiated myotubes. (i,j) GFP-Rbm24RRM was uniformly distributed in proliferating myoblasts and in multinucleated myotubes. Scale bars: 10 µm.
