Table 3 Repeatability of Biomeme and Precision Biomonitoring TripleLock SARS-CoV-assay.

From: Evaluation of mobile real-time polymerase chain reaction tests for the detection of severe acute respiratory syndrome coronavirus 2

(A)

All targets across individual runs, technicians, and Franklin

Target

Technician 1

Technician 2

Technician 3

Ist run

2nd run

1st & 2nd run

Ist run

2nd run

1st & 2nd run

Ist run

2nd run

1st & 2nd run

Franklin 1

Orf1ab gene

30.62 ± 1.1 (9.8%)

31.38 ± 0.9 (8.0%)

31.00 ± 0.7 (8.8%)

27.17 ± 0.5 (5.6%)

26.09 ± 0.6 (7%)

26.90 ± 0.4 (6.4%)

27.01 ± 0.8 (8.4%)

27.83 ± 1.5 (15.7%)

27.42 ± 0.8 (12.3%)

S gene

26.78 ± 0.5 (5.7%)

27.03 ± 1.9 (7.1%)

26.90 ± 0.4 (6.2%)

25.61 ± 0.2 (2.7%)

24.99 ± 0.3 (3.9%)

25.30 ± 0.2 (3.5%)

26.88 ± 0.4 (4.5%)

26.39 ± 0.6 (6.6%)

26.63 ± 0.4 (5.5%)

RNA process control

30.88 ± 1.2 (11.3%)

29.91 ± 1.0 (9.0%)

30.43 ± 0.8 (10.0)

30.70 ± 1.2 (9.9%)

28.35 ± 1.2 (11.8%)

29.53 ± 0.9 (11.0)

30.69 ± 0.9 (8.2%)

29.91 ± 2.0 (18.4%)

30.30 ± 1.1 (13.6)

Franklin 2

Orf1ab gene

29.40 ± 1.2 (11.4%)

29.02 ± 1.0 (9.8%)

29.21 ± 0.8 (10.3%)

25.72 ± 0.5 (6.0%)

25.26 ± 0.7 (7.8%)

25.49 ± 0.4 (6.8%)

26.20 ± 0.8 (9.0%)

26.31 ± 0.6 (6.7%)

26.25 ± 0.5 (7.7%)

S gene

26.22 ± 0.8 (9.0%)

26.48 ± 0.4 (4.6%)

26.35 ± 0.5 (7.0%)

25.46 ± 0.4 (5.0%)

25.02 ± 0.4 (5.0%)

25.24 ± 0.3 (4.9%)

26.69 ± 0.6 (5.9%)

26.14 ± 0.5 (5.0%)

26.41 ± 0.4 (5.4%)

RNA process control

29.54 ± 1.5 (13.2%)

30.31 ± 1.3 (12.5%)

29.95 ± 1.0 (12.4%)

29.55 ± 1.1 (10.2%)

29.03 ± 1.0 (10.2%)

29.29 ± 0.7 (9.9%)

30.71 ± 1.0 (8.3%)

29.39 ± 0.7 (6.7%)

30.05 ± 0.6 (7.7%)

Franklin 3

Orf1ab gene

29.30 ± 1.0 (9.8%)

30.07 ± 0.9 (8.0%)

29.68 ± 0.6 (8.7%)

25.79 ± 0.6 (6.4%)

25.10 ± 0.7 (8.1%)

25.45 ± 0.5 (7.2%)

26.77 ± 0.8 (8.1%)

27.03 ± 0.8 (8.8%)

26.90 ± 0.6 (8.2%)

S gene

26.34 ± 0.6 (6.4%)

26.59 ± 0.6 (6.0%)

26.46 ± 0.4 (6.0%)

26.33 ± 0.7 (8.0%)

25.25 ± 0.5 (6.0%)

25.79 ± 0.5 (7.1%)

26.28 ± 0.5 (5.5%)

26.12 ± 0.6 (6.9%)

26.20 ± 0.4 (6.0%)

RNA process control

30.19 ± 0.9 (7.6%)

30.67 ± 1.4 (12.6%)

30.45 ± 0.8 (10.3%)

30.80 ± 1.2 (11.0%)

28.13 ± 0.8 (8.5%)

29.47 ± 0.8 (10.6%)

29.72 ± 0.8 (7.1%)

30.47 ± 1.3 (11.7%)

30.12 ± 0.7 (9.6%)

(B)

      

All targets across the three Franklin

      

Target

Franklin 1

Franklin 2

Franklin 3

      

Orf1ab gene

28.35 ± 0.5 (11.5%)

26.98 ± 0.4 (10.3%)

27.34 ± 0.4 (10.3%)

      

S gene

26.28 ± 0.2 (5.8%)

26.00 ± 0.2 (6.1%)

26.15 ± 0.2 (6.3%)

      

RPC

30.09 ± 0.5 (11.4%)

29.75 ± 0.4 (10.1%)

30.00 ± 0.4 (10.1%)

      

(C)

        

All targets in the three Franklin combined

        

Target

Franklin 1, 2, & 3

        

Orf1ab gene

27.56 ± 0.2 (10.9%)

        

S gene

26.14 ± 0.1 (6.1%)

        

RPC

29.94 ± 0.3 (10.4%)

        

(D)

     

Day

 

5′UTR gene

E gene

RNaseP

     

1

Mean

28.91

26.84

28.91

     

SD

0.528507

0.528507

0.528507

     

SEM

0.141

0.137

0.134

     

CV

1.892

1.975

1.903

     

2

Mean

28.67

28.67

28.67

     

SD

0.453346

0.453346

0.453346

     

SEM

0.121

0.023

0.137

     

CV

1.627

0.331

1.945

     

3

Mean

29.07

29.07

29.07

     

SD

0.318276

0.318276

0.318276

     

SEM

0.085

0.040

0.136

     

CV

1.133

0.575

1.925

     

4

Mean

29.28

29.28

29.28

     

SD

0.450843

0.450843

0.450843

     

SEM

0.120

0.114

0.125

     

CV

1.594

1.636

1.748

     

5

Mean

29.19

29.19

29.19

     

SD

0.458395

0.458395

0.458395

     

SEM

0.123

0.086

0.113

     

CV

1.625

1.242

1.570

     

(E)

    
  

Mean

SD

SEM

%CV

    

Franklin D13AB6A254B2

5′UTR gene

28.98

0.374797

0.077

1.32

    

E Gene

26.88

0.336693

0.069

1.279

    

RNaseP

27.28

0.472211

0.096

1.766

    

Franklin

CC92BD2871DB

5′UTR gene

29.06

0.588501

0.12

2.067

    

E Gene

26.95

0.201179

0.041

0.762

    

RNaseP

27.39

0.478864

0.098

1.785

    

Franklin C43440902003

5′UTR gene

29.03

0.501677

0.102

1.764

    

E Gene

26.96

0.442525

0.09

1.675

    

RNaseP

27.76

0.492146

0.1

1.81

    
  1. The M1 Sample Prep Cartridge Kit for RNA 2.0 was used to separately extract RNA from cell culture-amplified SARS-CoV-2. Following RNA extraction, three technicians ran the Biomeme SARS-CoV-2 Test (twice) with eight extracts of the same concentration on three different Franklin three9. The mean and standard error of the mean (SEM) of the Ct values from the runs, in addition to the coefficient of variation (CV, shown in brackets), are shown. Tables show the individual and combined runs of the three technicians across the three Franklin three9 (A), the combined runs of the three technicians across the three Franklin three9 (B), and the combined runs of the three technicians and the three Franklin three9 (C). Separate extractions were conducted for the Precision Biomonitoring SARS-CoV-2 test on five separate days across three thermocyclers, the variation in Ct values was described across the across days (D) and the three Franklin three9 (E).
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