Figure 3 | Scientific Reports

Figure 3

From: Estrogen withdrawal alters cytoskeletal and primary ciliary dynamics resulting in increased Hedgehog and osteoclastogenic paracrine signalling in osteocytes

Figure 3

The effect of estrogen withdrawal on the role of the actin cytoskeleton in primary cilium dynamics. (A) Immunocytochemistry images showing acetylated α-tubulin, actin cytoskeleton, and nuclei staining (N = 3, n ≥ 92 cells per group). Quantification of the images showing (B) cell area, (C) actin fluorescent intensity, and (D) anisotropy of actin fibres. Quantification of the images to determine trend between primary cilium length and (E) cell area, (F) actin fluorescent intensity, and (G) anisotropy of actin fibres. (Student’s t-test, **p < 0.01, ****p < 0.0001. One-way ANOVA to determine trend between groups, #p < 0.05).

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