Table 1 Measured values of wavelength shifts by various combinations of Streptavidin solutions with flow methods in each testing procedure and calculated ratios by Δλ2/Δλ1 as described in Fig. 6b.
Flow types | ~ 5 min | ~ 15 min | ~ 20 min | Ratio (Δλ2/Δλ1) | |||
|---|---|---|---|---|---|---|---|
Solution | Δλ | Solution | Δλ1 | Solution | Δλ2 | ||
SF | 190 nM of SA | 99.7 pm | PBS | 98 pm | 190 nM of biotin-TNFα | 53.5 pm | 0.54 |
RFF | 0.19 fM of SA | 1400 pm | PBS | 239.4 pm | 190 nM of biotin-TNFα | 127.4 pm | 0.53 |
RFF | 1.9 fM of SA | 1425.7 pm | PBS | 872.7 pm | 190 nM of biotin-TNFα | 465.3 pm | 0.53 |
