Figure 1

The extrinsic incubation period of Plasmodium falciparum estimated using classical dissection approaches (a–d) and a novel non-destructive assay (e). (a) Proportion of infected mosquitoes with ruptured oocysts (± 95% CI) from 8 to 16 dpbm, expressed as the number of mosquitoes with at least one ruptured oocyst out of the total number of infected mosquitoes (i.e. harboring either intact and/or ruptured oocysts) for two parasite isolates. The lines represent best-fit logistic growth curves for each isolate. (b) Proportion of ruptured oocysts (± 95% CI), expressed as the number of ruptured oocysts out of the total number of oocysts (intact + ruptured). The lines represent best-fit logistic growth curves for each isolate. (c) Proportion of oocyst-infected mosquitoes with microscope-identified sporozoites in the salivary glands (± 95% CI), expressed as the number of oocyst-infected mosquitoes harboring sporozoites in their salivary glands out of the total number of infected mosquitoes. The lines represent best-fit logistic growth curves for each isolate. (d) Same as (c) but the presence of sporozoites was detected using qPCR. (a–d) Sample size = 8 to 20 midguts /day/isolate (median = 14). (e) Kaplan–Meier curves representing the temporal dynamics of sporozoites appearance in small pieces of cotton used to collect saliva from individual mosquitoes.