Figure 2 | Scientific Reports

Figure 2

From: The heat shock response, determined by QuantiGene multiplex, is impaired in HD mouse models and not caused by HSF1 reduction

Figure 2

Validation of QuantiGene multiplex assay to measure the expression of heat shock genes in mouse brain hemispheres and tibialis anterior muscle. Increase in the expression levels of Hsf1, Hspa1a/b, Hspb1 and Dnajb1 in wild-type mice was determined by QuantiGene (8-plex) (top graphs) and qPCR (bottom graphs) after treatment with NVP-HSP990 as compared to vehicle. The induction of the heat shock response resulted in an increase in the expression level of Hspa1a/b, Hspb1 and Dnajb1, but not in Hsf1 as would be expected. There was no difference in the fold changes in gene expression as measured by QuantiGene and qPCR. n = 4–6/treatment. Statistical analysis was by unpaired Student’s t-test. Mean ± SEM. ***p ≤ 0.001. Test statistics can be found in Supplementary Table S7 online. WT wild-type.

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