Figure 4
Analysis of the heat shock response in the striatum of zQ175 mice with disease progression. The expression of heat shock genes was measured using the QuantiGene 16-plex assay in the striatum of wild-type and zQ175 mice at 3, 12 and 20 months of age that had been treated with vehicle or NVP-HSP990. The NVP-HSP990 treated samples were normalised to the corresponding age-matched wild-type vehicle treated samples. For simplicity, the wild-type vehicle samples and the zQ175 vehicle samples from the three ages were combined. Five NVP-HSP990 treated samples were excluded from the analysis, because the tibialis anterior heat shock gene expression levels were comparable to those treated with vehicle. These were: at 3 months, two wild type; at 12 months, one wild type; at 20 months, one wild-type and one zQ175. N = 4–6/genotype/treatment/age. Statistical analysis was by two-way ANOVA with Bonferroni correction for multiple comparisons. Mean ± SEM. ***p ≤ 0.001. Test statistical values can be found in Supplementary Table S10 online. WT wild-type, m months.
