Figure 1
From: Direct conversion of porcine primary fibroblasts into hepatocyte-like cells
MOI determination for hepatic conversion of PKFs. (A) Schematic drawing of lentivirus vector design and cell culture protocol for directed hepatic conversion. (B) Albumin secretion quantification and (C) hepatic markers expression levels of cells directly reprogrammed with different MOIs per lentiviral vector of all 12 TFs, compared to Neg-Ctrl. All data were analyzed using two-way ANOVA, with Bonferroni’s post-test, except for TF gene, that was converted to mixed effects with Tukey’s multiple comparison post-test due to be missing one value. Significance from n = 3 independent values is displayed as *p < 0.05; ****p ≤ 0.0001.
