Figure 3 | Scientific Reports

Figure 3

From: Nanaomycin K inhibited epithelial mesenchymal transition and tumor growth in bladder cancer cells in vitro and in vivo

Figure 3

Gene expression of EMT markers induced by nanaomycin K in vitro. KK47 and T24 cells were treated with 5 µg/mL nanaomycin K with or without TGF-β in vitro for 48 h and the gene expressions of E-cadherin, N-cadherin and vimentin were determined by real time RT-PCR. Control cells were treated with DMSO (vehicle control) and mRNA levels were standardized by the expression levels of control gene β-actin (n = 3, average ± SE bars, *p < 0.05, **p < 0.01).

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