Figure 4
From: Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
Conversion of ethanol to 1,3-BDO. Results for implementation of the full system described in Fig. 1 are shown. The 1,3-BDO concentrations are shown in black and ethanol concentrations are shown in red. (a) Results for the full system including results leaving out formate or leaving out formate and NADP+. The ethanol consumption time course corresponds to the full system. (b) The same implementation except that extra ethanol and formate were added at various times (indicated by the red arrows). (c) The same implementation except that extra formate, ethanol, Nox and DERA enzymes were added at various times (indicated by the red arrows). Error bars reflect standard deviations of biological triplicates.
