Figure 2
Effect of SHM on apoptosis signaling in UVB-exposed human keratinocytes. (a) Apoptotic body formation in SHM-pretreated and UVB-exposed human keratinocytes. The cells were stained with Hoechst 33342 reagent (2 µg/mL) and examined using a fluorescence microscope. (b) Analysis of Sub-G1 apoptotic populations in SHM-pretreated and UVB-exposed human keratinocytes by flow cytometry using propidium iodide (PI). (c) The expression levels of mitochondria-mediated apoptotic molecules (Bcl-2, Bax, p53, and PARP) in SHM-pretreated and UVB-exposed human keratinocytes. (d) The density ratio of protein expression levels was analyzed via Image J software each in comparison to β-actin. Values are expressed as means ± SE of triplicate determinants (n = 3). a–eBars with different letters for each molecule represent significant differences (P < 0.01).
